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Ngày xuất bản:
21/09/2022
Ngày xuất bản Online:
21/09/2022
Số lượt xem tóm tắt: 206
Số lượt xem Chi tiết bài báo: 271
Số lượt xem Chi tiết bài báo: 271
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Trích dẫn bài báo
Nguyễn, N. T., & Nguyễn, T. D. (2022). Nghiên cứu sự thay đổi hàm lượng EGF, VEGF của bệnh nhân có vết thương mạn tính được điều trị bằng huyết tương giàu tiểu cầu. Tạp chí Y học Thảm hoạ và Bỏng, 4, 60-70. https://jbdmp.vn/index.php/yhthvb/article/view/137
Nghiên cứu sự thay đổi hàm lượng EGF, VEGF của bệnh nhân có vết thương mạn tính được điều trị bằng huyết tương giàu tiểu cầu.
Nội dung chính của bài viết
Tóm tắt
Vết thương mạn tính (VTMT) có sự rối loạn quá trình tái tạo và biểu mô hóa, một trong những nguyên nhân là có sự suy giảm các grow factor (GF). Huyết tương giàu tiểu cầu (platelet-rich plasma - PRP) điều trị vết thương mạn tính là một liệu pháp hiệu quả, an toàn cao, xâm lấn tối thiểu. Chúng tôi tiến hành nghiên cứu nhằm:
1 Đánh giá sự thay đổi hàm lượng EGF (epidermal growth factor) và VEGF (vascular endothelial growth factor) trong PRP trước và sau khi hoạt hóa.
2. Đánh giá sự thay đổi hàm lượng EGF, VEGF tại chỗ VTMT được điều trị bằng PRP.
Nghiên cứu tiến hành trên 24 bệnh nhân (BN) có VTMT, từ 18 tuổi trở lên, điều trị tại Bệnh viện Bỏng Quốc gia từ 11/2018 - 2/2020 bằng phương pháp tiêm PRP tại VTMT. Định lượng EGF và VEGF theo nguyên lý phản ứng Elisa, sử dụng chất chuẩn là EGF/VEGF và kháng thể đặc hiệu với EGF/VEGF.
Kết quả cho thấy sau hoạt hóa, hàm lượng EGF/VEGF trong PRP đều tăng; hàm lượng EGF và VEGF mô vết thương cũng tăng sau 1 tuần điều trị.
Kết luận: Liệu pháp PRP điều trị tại chỗ có tác dụng kích thích liền vết thương do đã làm tăng EGF và VEGF tại VTMT.
Chi tiết bài viết
Tài liệu tham khảo
1. Mathieu D., Linke J.C., Wattel F. “Non-healing wounds”. In: Handbook on hyperbaric medicine, Mathieu D.E. Netherlands: Springer, 2006, pp. 401-427.
2. Menke N.B., Ward K.R., Witten T.M., Bonchev D.G., Diegelmann R.F. “Impaired wound healing”. Clin Dermatol. 2007, 25:19-25
3. Dowsett C. “Breaking the cycle of hard-to-heal wounds: balancing cost and care”. Wounds International 2015, Vol 6 Issue 2.
4. Marta Otero-Viñas and Vincent Falanga. “Mesenchymal Stem Cells in Chronic Wounds: The Spectrum from Basic to Advanced Therapy”. Adv Wound Care (New Rochelle) 2016, Apr 1; 5(4): 149-163.
5. Banfi G. “Platelet-rich plasma”. Journal of biological regulators and homeostatic agents 2012;26, 1.
6. Amable P.R., Carias, R.B., Teixeira, M.V., da Cruz Pacheco, I., Correa do Amaral R.J., Granjeiro J.M. and Borojevic R. “Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors”. Stem cell research & therapy, 2013, 4 (3): 67
7. Elena Conde-Montero, Pablo de la Cueva Dobao, José María Martínez González. “Platelet-rich plasma for the treatment of chronic wounds: evidence to date”. Chronic Wound Care Management and Research. 2017, Vol 2017:4 107-120
8. Krister Järbrink, Gao Ni, Henrik Sönnergren, Artur Schmidtchen, Caroline Pang, Ram Bajpai and Josip Ca. “Prevalence and incidence of chronic wounds and related complications: a protocol for a systematic review”. Syst Rev. 2016; 5(1): 152
9. National Pressure Ulcer Advisory Panel (NPUAP); Pressure Injury Stages, http://www.npuap.org, 2016.
10. Vladimir N. Obolenskiy, Darya A. Ermolova, Leonid A. Laberko. “Efficacy of platelet-rich plasma for the treatment of chronic wounds”. The EWMA Journal, volume 14, No 1, April 2014, p. 37-40.
11. Mehrannia M., Vaezi M., Yousefshahi F., Rouhipour N. “Platelet-rich plasma for treatment of nonhealing diabetic foot ulcers: a case report”. CJD (Canadian journal of diabetes), February 2014, Volume 38, Issue 1, Pages 5 - 8.
12. Javier Ramos-Torrecillas, Elvira De Luna-Bertos, Olga García-Martínez. “Clinical Utility of Growth Factors and Platelet-Rich Plasma in Tissue Regeneration: A Review”. Wounds. 2014; 26(7):207-213
13. Diegelmann R.F. and Evans M.C. “Wound Healing: An Overview of acute, fibrotic and delayed healing”. Frontiers in Bioscience 9, 283-289
14. George Han and Roger Ceilley.“Chronic Wound Healing: A Review of Current Management and Treatments”. Adv Ther. 2017; 34(3): 599–610.
15. Sabine Werner and Richard; Regulation of Wound Healing by Growth Factors and Cytokines; Gros Physiol Rev 83: 835–870, 2003;
16. Stephan Barrientos; Olivera Stojadinovic, Michael S. Golinko, Harold Brem, Marjana Tomic-Canic (2008), Growth factors and cytokines in wound healing; Wound Repair and RegenerationWound, (2008), 16, 585–601
17. Hart J.Inflammation. 1: Its role in the healing of acute wounds. J Wound Care. 2002 Jun; 11(6):205-9.
18. Zhang Y, Wang T, Dong J (2016); Growth factor therapy in patients with partial-thickness burns: a systematic review and meta-analysis. Wound J. 2016 Jun; 13(3): 354-66.
19. Sho Yamakawa & Kenji Hayashida. Advances in surgical applications of growth factors for wound healing; Burns & Trauma, Volume 7, Article number: 10 (2019).
20. Park JW, Hwang SR, Yoon IS. Advanced growth factor delivery systems in wound management and skin regeneration. Molecules. 2017; 22:E1259.
21. Nanney LB. Epidermal and dermal effects of epidermal growth factor during wound repair. J Invest Dermatol. 1990; 94:624–9.
22. J.Hardwicke, D.Schmaljohann, D.Boyce, D.Thomas; Epidermal growth factor therapy and wound healing — past, present and future perspectives; The SurgeonVolume 6, Issue 3, June 2008, Pages 172-177
23. Brown GL, Curtsinger LJ, White M, Mitchell RO, Pietsch J, Nordquist R, et al. Acceleration of tensile strength of incisions treated with EGF and TGF-beta. Ann Surg. 1988; 208:788–94.
24. Kelly E. Johnson and Traci A. Wilgus (2014); Vascular Endothelial Growth Factor and Angiogenesis in the Regulation of Cutaneous Wound Repair; Adv Wound Care. 2014 Oct 1; 3(10): 647–661.
25. Thittamaranahalli Muguregowda Honnegowda, Pramod Kumar, Echalasara Govindarama Padmanabha Udupa, Sudesh Kumar, Udaya Kumar, Pragna Rao (2015); Role of angiogenesis and angiogenic factors in acute and chronic wound healing; Plast Aesthet Res 2015; 2:.243-249
26. Tatiana N. Demidova-Rice, Lindsey Wolf, Jeffry Deckenback, Michael R. Hamblin, Ira M. Herman (2012). “Human Platelet-Rich Plasma- and Extracellular Matrix-Derived Peptides Promote Impaired Cutaneous Wound Healing In vivo”. PLOS. https://doi.org/10.1371/journal.pone.0032146
2. Menke N.B., Ward K.R., Witten T.M., Bonchev D.G., Diegelmann R.F. “Impaired wound healing”. Clin Dermatol. 2007, 25:19-25
3. Dowsett C. “Breaking the cycle of hard-to-heal wounds: balancing cost and care”. Wounds International 2015, Vol 6 Issue 2.
4. Marta Otero-Viñas and Vincent Falanga. “Mesenchymal Stem Cells in Chronic Wounds: The Spectrum from Basic to Advanced Therapy”. Adv Wound Care (New Rochelle) 2016, Apr 1; 5(4): 149-163.
5. Banfi G. “Platelet-rich plasma”. Journal of biological regulators and homeostatic agents 2012;26, 1.
6. Amable P.R., Carias, R.B., Teixeira, M.V., da Cruz Pacheco, I., Correa do Amaral R.J., Granjeiro J.M. and Borojevic R. “Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors”. Stem cell research & therapy, 2013, 4 (3): 67
7. Elena Conde-Montero, Pablo de la Cueva Dobao, José María Martínez González. “Platelet-rich plasma for the treatment of chronic wounds: evidence to date”. Chronic Wound Care Management and Research. 2017, Vol 2017:4 107-120
8. Krister Järbrink, Gao Ni, Henrik Sönnergren, Artur Schmidtchen, Caroline Pang, Ram Bajpai and Josip Ca. “Prevalence and incidence of chronic wounds and related complications: a protocol for a systematic review”. Syst Rev. 2016; 5(1): 152
9. National Pressure Ulcer Advisory Panel (NPUAP); Pressure Injury Stages, http://www.npuap.org, 2016.
10. Vladimir N. Obolenskiy, Darya A. Ermolova, Leonid A. Laberko. “Efficacy of platelet-rich plasma for the treatment of chronic wounds”. The EWMA Journal, volume 14, No 1, April 2014, p. 37-40.
11. Mehrannia M., Vaezi M., Yousefshahi F., Rouhipour N. “Platelet-rich plasma for treatment of nonhealing diabetic foot ulcers: a case report”. CJD (Canadian journal of diabetes), February 2014, Volume 38, Issue 1, Pages 5 - 8.
12. Javier Ramos-Torrecillas, Elvira De Luna-Bertos, Olga García-Martínez. “Clinical Utility of Growth Factors and Platelet-Rich Plasma in Tissue Regeneration: A Review”. Wounds. 2014; 26(7):207-213
13. Diegelmann R.F. and Evans M.C. “Wound Healing: An Overview of acute, fibrotic and delayed healing”. Frontiers in Bioscience 9, 283-289
14. George Han and Roger Ceilley.“Chronic Wound Healing: A Review of Current Management and Treatments”. Adv Ther. 2017; 34(3): 599–610.
15. Sabine Werner and Richard; Regulation of Wound Healing by Growth Factors and Cytokines; Gros Physiol Rev 83: 835–870, 2003;
16. Stephan Barrientos; Olivera Stojadinovic, Michael S. Golinko, Harold Brem, Marjana Tomic-Canic (2008), Growth factors and cytokines in wound healing; Wound Repair and RegenerationWound, (2008), 16, 585–601
17. Hart J.Inflammation. 1: Its role in the healing of acute wounds. J Wound Care. 2002 Jun; 11(6):205-9.
18. Zhang Y, Wang T, Dong J (2016); Growth factor therapy in patients with partial-thickness burns: a systematic review and meta-analysis. Wound J. 2016 Jun; 13(3): 354-66.
19. Sho Yamakawa & Kenji Hayashida. Advances in surgical applications of growth factors for wound healing; Burns & Trauma, Volume 7, Article number: 10 (2019).
20. Park JW, Hwang SR, Yoon IS. Advanced growth factor delivery systems in wound management and skin regeneration. Molecules. 2017; 22:E1259.
21. Nanney LB. Epidermal and dermal effects of epidermal growth factor during wound repair. J Invest Dermatol. 1990; 94:624–9.
22. J.Hardwicke, D.Schmaljohann, D.Boyce, D.Thomas; Epidermal growth factor therapy and wound healing — past, present and future perspectives; The SurgeonVolume 6, Issue 3, June 2008, Pages 172-177
23. Brown GL, Curtsinger LJ, White M, Mitchell RO, Pietsch J, Nordquist R, et al. Acceleration of tensile strength of incisions treated with EGF and TGF-beta. Ann Surg. 1988; 208:788–94.
24. Kelly E. Johnson and Traci A. Wilgus (2014); Vascular Endothelial Growth Factor and Angiogenesis in the Regulation of Cutaneous Wound Repair; Adv Wound Care. 2014 Oct 1; 3(10): 647–661.
25. Thittamaranahalli Muguregowda Honnegowda, Pramod Kumar, Echalasara Govindarama Padmanabha Udupa, Sudesh Kumar, Udaya Kumar, Pragna Rao (2015); Role of angiogenesis and angiogenic factors in acute and chronic wound healing; Plast Aesthet Res 2015; 2:.243-249
26. Tatiana N. Demidova-Rice, Lindsey Wolf, Jeffry Deckenback, Michael R. Hamblin, Ira M. Herman (2012). “Human Platelet-Rich Plasma- and Extracellular Matrix-Derived Peptides Promote Impaired Cutaneous Wound Healing In vivo”. PLOS. https://doi.org/10.1371/journal.pone.0032146