Nguyên cứu sự biến đổi vi khuẩn và một số chỉ số hình thái mô tại chỗ vết thương thực nghiệm được điều trị bằng Polyhexamethylene Biguanide Hydrochloride

Abstract

Objective: To evaluate bacterial changes and certain local histomorphology parameters in experimental wounds treated with Polyhexamethylene Biguanide Hydrochloride (PHMB).
Subjects and methods: A controlled prospective experimental study was conducted on 10 New Zealand White rabbits, each with two symmetrical wounds created on their backs. Wound area A was treated with PHMB, and area B was treated with Silver Sulfadiazine 1%. Bacterial culture positivity rate, bacterial density per 1 cm² of wound, and selected histomorphology parameters were assessed at baseline (T0), day 5 (T1), and day 10 (T2).
Results: The bacterial culture positivity rate in area A decreased from 60% to 33.3% by day 10. Bacterial density in area A was higher than in area B (78.7 ± 7.2 vs. 35.7 ± 5.2 × 5×10³/cm², p < 0.05). The number of fibroblasts and neovascularization in area A increased significantly compared to area B (p < 0.05). The number of inflammatory cells increased on day 5 and decreased by day 10, with no significant difference between the two areas (p > 0.05).
Conclusion: PHMB effectively controls bacterial load and promotes fibroblast proliferation and neovascularization in wounds, thereby contributing to improved healing.