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Date Published: 28/08/2025
Online Published: 28/08/2025
Abstract Views: 181
Views article details: 9
DOI: https://doi.org/10.54804/yhthvb.4.2025.480
Issue
No. 4 (2025)
Section
Articles
How to Cite
Ngo, M. D., Chu, A. T., Do, X. H., Tuong, P. V., Le, T. T., & Vu, A. N. (2025). Ultrastructural characteristics of Doxorubicin induced skin tissues treated with human umbilical cord blood platelet-rich plasma. Journal of Disaster Medicine and Burn Injuries, 4, 100-109. https://doi.org/10.54804/yhthvb.4.2025.480

Ultrastructural characteristics of Doxorubicin induced skin tissues treated with human umbilical cord blood platelet-rich plasma

Ngo Minh Duc1,2,, Chu Anh Tuan1, Do Xuan Hai3, Tuong Phi Vuong4, Le Tai The4, Vu Anh Ngu4
1 Le Huu Trac National Burn Hospital
2 Vietnam Medical Military University
3 Vietnam Military Medical University
4 Institute 69, President Ho Chi Minh Mausoleum Command

Main Article Content

Abstract

Objective: Evaluation of ultrastructural characteristics (USC) of chronic wounds (CW) induced with doxorubicin and changes in USC of CW treated with human umbilical cord blood platelet-rich plasma (PRP).
Subjects and methods: Chronic wounds were induced in 8 rats by doxorubicin injection, the day of CW formation was counted as D0, samples were taken from 2 rats to evaluate the USC of the CWs. The remaining rats, that had CW, were divided into 2 groups: the control group (treated with NaCl 0,9%): 3 rats were sampled at D14, D21 and recovered; the study group was injected with PRP at D5 and D10, sampled at D14, D21 and recovered. The samples were analyzed under a JEOL JEM 1400 transmission electron microscope (TEM) and a JSM 5410LV scanning electron microscope (SEM) (Japan).
Results: Ulcers created by doxorubicin injection on rat skin tissue on day 12 had the same USC as CW. CW treated with PRP had a faster wound healing process than the control group: early inflammatory cell infiltration, more neovascularization, more infiltration of fibroblasts and like mesenchymal cells, the epithelialization process occurred faster.
Conclusion: On the 12th day after doxorubicin injection, the skin ulcer in rats had an image similar to a chronic wound. In chronic wound, human umbral cord blood PRP had anti-inflammatory effects, increased connective tissue regeneration, and better angiogenesis, leading to faster wound healing time compared to treatment with only 0.9% NaCl.

Article Details

Keywords

Chronic wounds, TEM, SEM, fibroblasts, collagen, Doxorubicin, PRP

References

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