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The clinical effectiveness of Berberine nano gel on wound healing in rabbits.
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Abstract
Berberine is isolated from the plant Coscinium fenestratum and some plants in the family Ranunculaceae have affected wound healing and burn to heal. The research to determine the repair of full skin loss wounds of rabbits with Berberine nano gel produced by the National Burn Hospital.
Conducted on 45 rabbits, creating a skin loss of 3.5cm of diameter on each side of the rabbit's back (2 wounds/rabbit), rabbits were separated into 3 groups (15 rabbits, 30 wounds/each group).
+ Research group (Group A): Topical treatment with Berberine nano gel.
+ Control group (Group B): Topical treatment with SSD 1%.
+ Standard group (Group C): Topical treatment with 0.9% Sodium Chloride.
Keeping track of local and systemic symptoms. Results: There was no local or systemic allergic or sensitization; exudation and wound inflammation decreased more in Group A than in Groups B and C; the number of wound healing in Group A between 2 weeks and 3 weeks was higher than in Group B and Group C; The rate of re-epithelialization and the speed of epithelialization of Group A after 2 weeks were also higher than other groups.
Conclusion: Berberine nano gel for treating skin loss wounds has been demonstrated in the study to be safe, anti-inflammatory, bacteria inhibiting, and wound healing stimulating.
Article Details
Keywords
Berberine nano gel, abbit, rabbit, skin loss wound
References
2. Stephens P, Caley M, Peake M. Alternatives for animal wound model systems. In: RG Gourdie, TA Myers, eds. Wound Regeneration and Repair Methods and Protocols. New York, NY: Humana Press; 2013: 177-201.
3. Stojadinovic O, Tomic-Canic M. Human ex vivo wound healing model. In: RG Gourdie, TA Myers, eds. Wound Regeneration and Repair Methods and Protocols. New York, NY: Humana Press; 2013: 255-264.
4. Daniela S. Masson-Meyers, Thiago AM Andrade, Guilherme F Caetano, Francielle R Guimaraes, Marcel N Leite, Saulo N Leite, Marco Andrey C Frade, Experimental models and methods for cutaneous wound healing assessment. Int J Exp Pathol, 2020. 101(1-2): p. 21-37
5. Gottrup F, Agren MS, Karlsmark T. Models for use in wound healing research: a survey focusing on in vitro and in vivo adult soft tissue. Wound Repair Regen. 2000; 8: 83- 96
6. Nguyễn Ngọc Tuấn, Nghiên cứu quy trình sản xuất dung dịch berberin và kem berberin, đánh giá tác dụng điều trị của chế phẩm tại vết thương bỏng, đề tài nghiên cứu cấp Sở Khoa học và Công nghệ Hà Nội, 2011
7. OECD guideline for the testing of chemicals, 402, 2017
8. Wang XQ, Liu PY, Kempf M, Cuttle L, Mill J, Kimble RM. Burn wound infection in a porcine burn model. J Burn Care Res 2009;30 (2):369-70.
9. Kemal Arslan, Omer Karahan, Ahmet Okuş, Yaşar Unlü, Mehmet Ali Eryilmaz, Serden ay, Barış Sevinç; Comparison of topical zinc oxide and silver sulfadiazine in burn wounds: an experimental study; Turkish Journal of Trauma & Emergency Surgery, 2012;18 (5):376-383
10. Li N, G.L., Linlin Q, et al., Berberine attenuates pro-inflammatory cytokine-induced tight junction disruption in an in vitro model of intestinal epithelial cells. European Journal of Pharmaceutical Sciences, 2010. 40(1): p. 1-8.
11. Kuo CL, C.C., Liu TY; The anti-inflammatory potential of berberine in vitro and in vivo. Cancer, 2004. 20(203(2): p. 127-37
12. Wu J.F., L.T.P., Effects of berberine on platelet aggregation and plasma levels of TXB2 and 6-keto-PGF1 alpha in rats with reversible middle cerebral artery occlusion. Yao Hsueh Hsueh Pao, 1995. 30: p. 98-102
13. Ang E.S.W., L.S.T., Gan CSG, Evaluating the role of alternative therapy in burn wound management: randomized trial comparing moist exposed burn ointment with conventional method in the management of patients with second-degree burns, Medscape general medicine, 2001. 3(12): p. 3-18.
14. Huang CG, C.Z.L., Yang ZM, Effects of berberine on synthesis of platelet TXA2 and plasma PGI2 in rabbits. Chung Kuo Yao Li Hsueh Pao, 1991. 12: p. 526-528
15. Sabir M, A.M., Bhide NK; Further studies on pharmacology of berberine. Indian J Physiol Pharmacol; 1978. 22: p. 9-23
16. Jeong HW, H.K.C., Lee JW, et al., Berberine suppresses pro-inflammatory responses through AMPK activation in macrophages, Am J Physiol Endocrinol Metab; 2009. 296: p. e955-e964
17. Hu X, Z.Y., Xue Y, Zhang Z, Wang J, Berberine is a potential therapeutic agent for metabolic syndrome via brown adipose tissue activation and metabolism regulation. Am. J. Transl. Res., 2018. 10: p. 3322-3329
18. Timothy C, B.N.D., Gregory ND, Berberine: Therapeutic potential of an Alkaloid found in several medicinal plant,. Alt Med Rev, 1997. 2(2): p. 94-103
19. Amin AH, S.T.V., Abbasi KM; Berberine sulfate: antimicrobial activity, bioassay, and mode of action, Can J Microbiol, 1969. 15: p. 1067-1076.
20. Stermitz FR, L.P., Tawara JN et al., Synergy in a medicinal plant: Antimicrobial action of berberine potentiated by 5′-methoxyhydnocarpin, a multidrug pump inhibitor, Proc Natl Acad Sci USA, 2000. 97(4): p. 1433-1437.
21. Yu HH, K.K.J., Cha JD et al., Antimicrobial Activity of Berberine Alone and in Combination with Ampicillin or Oxacillin Against Methicillin-Resistant S. aureus, Journal of Medicinal Food., 2005. 8(4): p. 454-461.
22. Košťálová; M.Č.D., Antimicrobial activity of berberine - a constituent of Mahonia aquifolium; Folia Microbiologica; 2002. 47(4): p. 375-378
23. Gilbs PJ, SKR, Berberine. Alternative Medicine Review, 2000. 5(2): p. 175.
24. Sabir M, A.M., Bhide NK; Further studies on pharmacology of berberine. Indian J Physiol Pharmacol; 1978. 22: p. 9-23
25. Rui Zhou, Changpei Xiang, Guangzhao Cao, He Xu, Yi Zhang, Hongjun Yang, Jingjing Zhang; Berberine accelerated wound healing by restoring TrxR1/JNK in diabetes; Clin Sci (Lond). 2021 Feb 12;135(4):613-627.
26. Dibya Sundar Panda, Hussein M Eid, Mohammed H Elkomy, Ahmed Khames, Randa M Hassan, Fatma I Abo El-Ela, Heba A Yassin; Berberine Encapsulated Lecithin-Chitosan Nanoparticles as Innovative Wound Healing Agent in Type II Diabetes; Pharmaceutics. 2021 Aug 4;13(8):1197.
27. A Asif, G Kakub, Sajid Mehmood, Rizwana Khanum; Wound healing activity of root extracts of Berberis lyceum Royle in rats; Phytotherapy Research 21(6):589-91June 2007; DOI:10.1002/ptr.2110
28. Mansoureh Pashaee, Abdolhossein Shiravi; Vida Hojati; The Effect of Hydroalcoholic Extract of Berberis vulgaris on Wound Healing of Diabetic Wistar Rats; Journal of chemical health risks; Volume 6, Issue 4 - Serial Number 4; 42; September 2016; DOI: 10.22034/JCHR.2016. 544159
Qian Li Tang; Shan Shan Han; Jing Feng; Jia Qi Di; Wen Xi Qin; Jun Fu; Qiu Yan Jiang, Moist exposed burn ointment promotes cutaneous excisional wound healing in rats involving VEGF and bFGF, Molecular Medicine Reports; April 2014; Volume 9 Issue 4, Pages: 1277-1282