Phương pháp định lượng hình thái đánh giá kết quả nhuộm hóa mô miễn dịch bộc lộ dấu ấn kháng nguyên CD31, CD34 trên tế bào nội mô vi mạch mô da sau xạ trị.

Abstract

Skin damage is one of the most frequent side effects during radiotherapy in patients with cancer diseases. In this study, the quantitative morphological method was used to assess the level of CD31 and CD34 expression on the skin microvascular endothelial cells after radiotherapy.
We collected thirty radiation-ulcer skin samples, each of which has three studied regions: the center (zone 1), the affected (zone 2), and the outside (zone 3) of the radiation-induced skin ulcer. We found that, the surface density (Sv) and length density (Lv) of a blood vessel with CD31 and CD34 positive decreased statistic significantly from center to outside of the ulcer. We conclude that our developed morphological quantitative method could be used to evaluate the results of CD31 and CD34 expression on skin microvascular endothelial cells after radiotherapy. Moreover, the success of our study may permit the following application of this morphological quantitative method for analysis of further immunohistochemistry studies.