Đánh giá mô hình vết loét da mạn tính trên chuột cống trắng bằng Doxorubicin

Abstract

Objectives: Evaluate the characteristics and standards of chronic skin ulcers in rat models by injecting derma with different doses of Doxorubicin to select an appropriate model before evaluating the effectiveness of treatment methods.
Subjects and methods: Wistar rats (170 - 250 grams) were injected intradermally on both sides of the back with Doxorubicin (at a concentration of 2mg/ml) in doses of 0.2mg, 0.5mg, and 1mg at 3 different locations in the lower abdomen: Behind the shoulders and the ears. After the skin ulcer reaches its maximum, create a circular cut wound with a diameter of 10mm.
Results: 100% effective in forming skin ulcers. At injection doses of 0.2mg, 0.5mg and 1mg, the time for lesions to appear is 3.89 ± 0.74 days, 3.44 ± 0.50 days and 3.29 ± 0.70 days, respectively; The time to maximum damage was 7.00 ± 0.82 days, 11.50 ± 1.12 days and 15.50 ± 0.96, respectively. The wound healing time after ulcer creation was 12.5 ± 2.18 days, 23.25 ± 2.33 days and 58.00 ± 7.65 days, respectively. At injection doses of 0.5 mg and 1 mg, the lesions had characteristic chronic inflammation on histopathological testing.
Conclusion: To induce a model of chronic skin ulcers in rats by intradermal injection of Doxorubicin (2mg/ml), the injection dosage should be chosen: 0.5 - 1mg (0.25ml - 0.5ml)/site. Injection, at a location behind the front leg.