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Date Published: 28/06/2025
Online Published: 28/06/2025
Abstract Views: 213
Views article details: 63
DOI: https://doi.org/10.54804/yhthvb.3.2025.431
Issue
No. 3 (2025)
Section
Articles
How to Cite
Nguyen, T. C., Le, T. T., Bui, K. C., Dinh, T. T. X., & Do, X. H. (2025). Optimization of human umbilical artery decellularization using freeze- thaw cycles combined with low concentration SDS perfusion. Journal of Disaster Medicine and Burn Injuries, 3, 96-106. https://doi.org/10.54804/yhthvb.3.2025.431

Optimization of human umbilical artery decellularization using freeze- thaw cycles combined with low concentration SDS perfusion

Nguyen Trung Chu1,, Le Tai The, Bui Khac Cuong2, Dinh Thi Thanh Xuan3, Do Xuan Hai4
1 Vietnam Medcine Military University
2 108 Military Central Hospital
3 Vietnam Medicine Military University
4 Vietnam Medicne Military University

Main Article Content

Abstract

Objective: To optimize the decellularization protocol for human umbilical arteries (HUAs) by combining freeze-thaw cycles with low-concentration Sodium Dodecyl Sulfate (SDS) perfusion to create a biocompatible small-caliber vascular scaffold.
Subjects and methods: HUAs, after three freeze-thaw cycles, were divided into three groups: (Tx-0.3-A) mechanical shaking with 0.3% SDS after Triton X-100 immersion for 24 hours, (Tx-0.3-P) perfusion with 0.3% SDS after Triton X-100 immersion for 24 hours, and (1-P) perfusion with 1% SDS. Decellularization efficiency was evaluated using H&E and DAPI staining, DNA quantification; ECM preservation was assessed via Masson’s trichrome, Alcian Blue staining, and hydroxyproline assay; and mechanical properties were evaluated.
Results: The Tx-0.3-P group showed superior decellularization efficiency, with excellent preservation of collagen, GAGs, and mechanical properties (tensile strength 3232 ± 400 kPa; maximum strain 1.18 ± 0.05). The group with 1% SDS caused more ECM damage due to the high SDS concentration.
Conclusion: The 0.3% SDS perfusion following freeze-thaw preconditioning is the optimal protocol for generating small-caliber vascular scaffolds from HUAs, with promising potential for biomedical applications in vascular tissue regeneration.

Article Details

Keywords

Human umbilical artery, Decellularization, Extracellular matrix, Sodium dodecyl sulfate, Small-caliber blood vessels, Freeze-thaw

References

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